Journal: Frontiers in Immunology

Article Title: IgE Epitope Profiling for Allergy Diagnosis and Therapy – Parallel Analysis of a Multitude of Potential Linear Epitopes Using a High Throughput Screening Platform

doi: 10.3389/fimmu.2020.565243

Figure Lengend Snippet: Commercially available antibodies used in this study.

Article Snippet: , monoclonal, clone B3102E8 , AlexaFluor555 , Southern Biotech#9160-32.

Techniques:

Journal: Bioscience Reports

Article Title: The final proteolytic step in transmembrane signaling of multiple RsgI anti-σ factors in Clostridium thermocellum

doi: 10.1042/BSR20253055

Figure Lengend Snippet: (A ) SDS-PAGE of the eight purified RsgI-NFs samples. The expected RsgI-NF bands are indicated by red arrows. ( B ) Western blot analysis of the eight purified RsgI-NFs using the anti-His6-tag antibodies.

Article Snippet: These membranes were blocked using TBST-blocking buffer (50 mM Tris, 150 mM NaCl, 0.07% Tween-20, 5% skim milk, pH 7.4) for a duration of 2 h. Then, the membranes were incubated with mouse anti-His tag monoclonal antibody (Bioss Antibodies, Beijing, China) overnight at 4°C.

Techniques: SDS Page, Purification, Western Blot